Journal: Scientific Reports

Article Title: Single-cell transcriptomes reveal heterogeneity of chlorine-induced mice acute lung injury and the inhibitory effect of pentoxifylline on ferroptosis

doi: 10.1038/s41598-023-32093-7

Figure Lengend Snippet: PTX inhibit Cl 2 -induced ferroptosis. ( A , B ) The expression levels of MDA, GSH/GSSG in mice serum treated with Cl 2 , n = 8. ( C , D ) Protein levels of GPX4 and SLC7A11 in mice lung and serum treated with Cl 2 , n = 8. ( E ) GPX4 and SLC7A11 protein levels were determined by western blot analysis, and standardized to the expression of β-actin. Original scans of whole blots are shown in Supplementary information 5. ( F , G ) Immunofluorescence images were used to detect the expression of GPX4 in epithelial and endothelial cells, scale bars 50 μm, n = 6. ( H , I ) Immunofluorescence images were used to detect the expression of SLC7A11 in epithelial and endothelial cells, scale bars 50 μm, n = 6. Epithelial cell: CK8 (green), endothelial cells: Pecam1 (green), GPX4 (red), SLC7A11 (red), cell nuclear (blue). The data was shown as mean ± SEM, * P < 0.05, ** p < 0.01, p < 0.001, **** p < 0.0001, n = 8.

Article Snippet: WB was performed using the following primary antibodies: SLC7A11(orb100617, biorbyt); GPX4 (orb135590, biorbyt); β-Actin (orb625112, biorbyt).

Techniques: Expressing, Western Blot, Immunofluorescence

Journal: PLoS ONE

Article Title: S-Propargyl-Cysteine, a Novel Hydrogen Sulfide Donor, Inhibits Inflammatory Hepcidin and Relieves Anemia of Inflammation by Inhibiting IL-6/STAT3 Pathway

doi: 10.1371/journal.pone.0163289

Figure Lengend Snippet: (A) SPRC and NaHS markedly decreased serum IL-6 levels induced by LPS (n = 5). (B-D) Hepatic Il-6 , Tnfa mRNA levels and JAK2/STAT3 phosphorylation were induced by LPS, and suppressed by SPRC and NaHS. Moreover, PAG diminished the effects of SPRC (n = 5). (E-F) Densitometry analysis of Fig 2D. (G-H) Hepatic mRNA levels of Socs3 and Saa2 , two target genes of STAT3 (n = 5). Representative immunoblots are presented. Whole uncropped images of Fig 2D are shown in . Data are presented as the mean ± SEM. ### p < 0.001 compared with the control group; * p < 0.05, ** p < 0.01, *** p < 0.001, compared with the LPS group; @ p < 0.05, @@ p < 0.01, @@@ p < 0.001.

Article Snippet: Antibody to phospho-JAK2 (Tyr221) was acquired from Bioworld Technology, Louis Park, MN, USA.

Techniques: Western Blot

Journal: PLoS ONE

Article Title: S-Propargyl-Cysteine, a Novel Hydrogen Sulfide Donor, Inhibits Inflammatory Hepcidin and Relieves Anemia of Inflammation by Inhibiting IL-6/STAT3 Pathway

doi: 10.1371/journal.pone.0163289

Figure Lengend Snippet: (A) Serum IL-6 content was not significantly changed by SPRC (n = 7). (B) Hepatic Il-6 mRNA levels were suppressed by SPRC, though only 2-fold change was observed in the model group (n = 7). (C) SPRC successfully ameliorated hepatic JAK2/STAT3 phosphorylation in the turpentine model. (D-E) Densitometry analysis of . Representative immunoblots are presented. Whole uncropped images of Fig 4C are shown in . Data are presented as the mean ± SEM. # p < 0.05, ### p < 0.001 compared with the control group; * p < 0.05, ** p < 0.01, *** p < 0.001 compared with the model group.

Article Snippet: Antibody to phospho-JAK2 (Tyr221) was acquired from Bioworld Technology, Louis Park, MN, USA.

Techniques: Western Blot

Journal: Biochemical pharmacology

Article Title: The JAK1/2 inhibitor baricitinib suppresses eosinophil effector function and restricts allergen-induced airway eosinophilia.

doi: 10.1016/j.bcp.2021.114690

Figure Lengend Snippet: Fig. 2. Baricitinib and tofacitinib similarly inhibit eosinophil differentiation and JAK1/2 activation. Bone marrow cells were collected from the femurs and tibiae of three female BALB/c mice and cultured at 106/mL in media supplemented with 100 ng/mL SCF and 100 ng/mL FLT3L from day 0 to day 4. On day 4, media were replaced with media containing 10 ng/mL IL-5 supplemented with or without baricitinib (BARI, 200 nM) or tofacitinib (TOFA, 200 nM). (A) Total cells, (B) Siglec F positive cells and (C) CCR3 positive cells were enumerated by flow cytometry on the indicated days. On day 12 (D) JAK1 and (E) JAK2 phosphorylation was measured by flow cytometric intracellular staining. Data are shown as mean ± SEM from 3 independent experiments, ** P < 0.01, *** P < 0.005; (A-C) two-way ANOVA and (D and E) one- way ANOVA.

Article Snippet: On day 12, cells were processed with Fix & Perm (Nordic MUbio) and additionally stained with FITC anti-mouse phospho-JAK1 and JAK2 antibodies (both Biorbyt), evaluated by flow cytometry (BD FACSCanto II) and and analyzed by FlowJo 10.7.1 software.

Techniques: Activation Assay, Cell Culture, Flow Cytometry, Phospho-proteomics, Staining