Journal: Chemistry & biology

Article Title: Selecting agonists from single cells infected with combinatorial antibody libraries.

doi: 10.1016/j.chembiol.2013.04.012

Figure Lengend Snippet: Figure 5. Signal Transduction by Agonist Antibodies JAK2 was purified from cell lysates using affinity to anti-JAK2 agarose and its phosphorylation was detected with western blotting using anti- phosphotyrosine antibodies. Phosphorylation of STAT3, STAT5, Akt, and MAPK induced by agonist antibodies or rhTPO stimulation was detected with direct western blotting of cell lysates. See also Figure S6.

Article Snippet: The immunoprecipitates were analyzed with western blotting using anti-phospho-JAK2 (Tyr1007/1008) (C80C3) antibody (Cell Signaling Technology, catalog no. 3776) and anti-total JAK2 antibody (C-14) (Santa Cruz Biotechnology, catalog no. sc-34479).

Techniques: Transduction, Phospho-proteomics, Western Blot

Journal: iScience

Article Title: The mutation of NSUN5 R295C promotes preeclampsia by impairing decidualization through downregulating IL-11Rα

doi: 10.1016/j.isci.2024.108899

Figure Lengend Snippet:

Article Snippet: Phospho-JAK2-Y1007 Rabbit pAb , ABclonal , Cat. NO: Ap0917; RRID: AB_2863830.

Techniques: Enzyme-linked Immunosorbent Assay, RNA Immunoprecipitation, Software

Journal: Scientific Reports

Article Title: Single-cell transcriptomes reveal heterogeneity of chlorine-induced mice acute lung injury and the inhibitory effect of pentoxifylline on ferroptosis

doi: 10.1038/s41598-023-32093-7

Figure Lengend Snippet: PTX inhibit Cl 2 -induced ferroptosis. ( A , B ) The expression levels of MDA, GSH/GSSG in mice serum treated with Cl 2 , n = 8. ( C , D ) Protein levels of GPX4 and SLC7A11 in mice lung and serum treated with Cl 2 , n = 8. ( E ) GPX4 and SLC7A11 protein levels were determined by western blot analysis, and standardized to the expression of β-actin. Original scans of whole blots are shown in Supplementary information 5. ( F , G ) Immunofluorescence images were used to detect the expression of GPX4 in epithelial and endothelial cells, scale bars 50 μm, n = 6. ( H , I ) Immunofluorescence images were used to detect the expression of SLC7A11 in epithelial and endothelial cells, scale bars 50 μm, n = 6. Epithelial cell: CK8 (green), endothelial cells: Pecam1 (green), GPX4 (red), SLC7A11 (red), cell nuclear (blue). The data was shown as mean ± SEM, * P < 0.05, ** p < 0.01, p < 0.001, **** p < 0.0001, n = 8.

Article Snippet: WB was performed using the following primary antibodies: SLC7A11(orb100617, biorbyt); GPX4 (orb135590, biorbyt); β-Actin (orb625112, biorbyt).

Techniques: Expressing, Western Blot, Immunofluorescence